Tuberculosis/Mycobacteriology Isolates Testing
Tuberculosis/Mycobacteriology Isolate specimen submission, packaging and shipping
External laboratories may be charged a fee per test. Contact the IDOH Lab for specific cost.
- Organisms: Mycobacterium tuberculosis complex, Mycobacteria species.
- Submit a pure culture on appropriate media, such as an LJ slant, 7H10, or MGIT broth.
- Only one isolate per patient should be submitted.
- Isolates of MTBC should be submitted within (3) business days of isolation per the IDOH Communicable Disease Rule.
Isolate cultured on appropriate type of media (LJ/MGIT/7H10, etc.) in tube with screw-cap tightened.
- Handle all isolates of MTBC, or those suspected of being MTBC, at a biosafety level 3.
- Label each isolate with a minimum of the patient’s name, date of birth, and date of specimen collection, including a minimum of two unique identifiers on the tube.
- Verify that the expiration date on the media is acceptable and will continue to be acceptable once received at the IDOH Laboratory.
- Complete the LimsNet test request for Tuberculosis (Mycobacteriology Isolate), ensuring that the patient information on the isolate tube label matches the information on the form.
- Specimens lacking completed information will not be tested until the missing information has been provided.
Indiana Department of Health Laboratory
Attn: TB Lab
550 W. 16th St., Suite B
Indianapolis, IN 46202
- If an isolate is a known member of the Mycobacterium tuberculosis complex or is suspected to be in this group, then the isolate must be labeled and shipped as a Category A Infectious Substance and must be packaged in accordance with all federal shipping regulations.
- Complete the Shippers Declaration for Dangerous Goods Documentation and include at least three copies on the outside of the package.
- Tighten the sample culture tube cap. Wrap lab tape or parafilm around the tube cap to help prevent any leakage of the sample.
- Wrap this primary container with absorbent material. Place the primary container and absorbent material in the inner mailing container (secondary packaging) and tighten the cap securely.
- The LimsNet coversheet should be included external to the sealed inner container and together enclosed securely in a rigid outer shipping container which should be clearly labeled with the sender's and recipient's name/address and the name and phone number of the responsible party.
- The outside of the package must be clearly labeled with the Category A, Infectious Substance label, and with the designation "UN 2814 Infectious Substance."
- Petri plates should not be submitted as they are unacceptable for testing.
- Isolates may be transported at ambient temperature.
- Results will be reported by LimsNet.
- Results should be available within 1-16 business days.
- The test request should include the specific test being requested (identification, susceptibility testing, or genotyping).
- Identification testing is performed by PCR or MALDI-TOF, and drug susceptibility testing will be performed on newly identified MTBC patients upon request.
- Isolates may be forwarded to the CDC or a reference laboratory for additional testing. Cultures identified as MTBC will be forwarded to the Michigan Department of Health & Human Services Bureau of Laboratories for genotyping studies as required by the IDOH Communicable Disease Rule.