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Haemophilus influenzae

Isolates of Haemophilus influenzae are tested for species identification/confirmation as well as agglutination serotyping for subtype of species.

Invasive species of Haemophilus influenzae are required to be sent to the IDOHL by the IDOH Communicable Disease Rule 410 IAC 1-2.5-76(f).

IDOH Reference Microbiology Laboratory will perform the identification/confirmation and serotyping to assess vaccine efficacy and to establish the Indiana prevalence baseline of each serotype. Currently, the only serotype with an available vaccine is Haemophilus influenzae serotype b (Hib).

No cost associated with this testing.

  1. Isolate should be pure, viable, and cultured onto an organism-appropriate agar medium slant.
    1. The IDOHL recommends using GC agar for effective transport and subculturing of isolates.
    2. Using a different medium for isolates may not allow for suitable growth upon reception at the IDOHL.
    3. Isolates that cannot be grown or are shown to be overly contaminated/mixed will be cancelled and require resubmission.
  2. Isolates should only be from an invasive source (410 IAC 1-2.5-46).
  3. Only one isolate per patient should be submitted.
  4. Isolates should be sent in within three(3) days according to Indiana Communicable Disease Rule 410 IAC 1-2.5-76(f).
  5. Isolates must also be received within a timely manner due to the potential of Haemophilus influenzae isolates to lose the ability to produce a capsule over time, reducing the accuracy of serotyping.
  6. Temperature requirement: Ambient condition.

Appropriate agar medium slant in tube with screw-cap tightened or other similarly approved commercial transport medium. GC agar is the medium recommended by the IDOHL for shipping Haemophilus influenzae isolates.

Complete submission form on LimsNet and include a copy of the LimsNet coversheet with the sample.

  • Isolates WILL NOT be accepted unless properly entered into LimsNet.
  • Confirm the isolate container is appropriately labeled with suspected species, patient name, patient DOB, date of isolation and a unique identifier.
  • Confirm the information on the container is identical to the information entered into LimsNet.
  • Check expiration date of tube to ensure the product is acceptable and will continue to be acceptable once received at the IDOH Laboratory.
  1. Ship to:
    Indiana Department of Health Laboratories
    Attention: Reference Microbiology
    550 West 16th Street, Suite B
    Indianapolis, IN 46202
  2. Package according to Category B UN3373 triple contained in accordance with federal shipping regulations for infectious substances/diagnostic specimens.
  3. Tighten the specimen container tube caps.
  4. Label each specimen tube with suspected species, patient name, patient DOB, date of isolation and a unique identifier.
  5. Wrap each labeled, primary/specimen container tube with absorbent material. Place each primary container tube with absorbent material into the inner mailing container and tighten the cap securely.
  6. Completed submission/request form may then be wrapped around the sealed inner container and together placed securely into the outer shipping container.
  7. Clearly label the outer container with the sender's name/address and recipient's name/address.
  8. Do not send culture isolates on petri plates if submitting by mail.
  9. Transport Temperature: Ambient conditions.

Reporting Method: LimsNet and Fax.
TAT: Typically 3 to 7 business days.
Test Referral:  Isolates may be forwarded to the regional VPD laboratory or the CDC for additional testing.

  • DO NOT send a liquid suspension culture of Haemophilus influenzae as the specimen will lose its ability to serotype accurately in only a few hours.
  • Make sure all specimens are packaged appropriately. Sample containers that are broken will not be accepted and will need to be resubmitted.
  • IDOH Title 410 Communicable Disease Rule may be viewed here.
  • LimsNet submissions information may be viewed here.