Listeria Specimen Collection and Transport
- Specimen type: Pure viable culture on appropriate agar medium slant.
- One isolate per patient.
- IDOH Communicable Disease Rule 410-IAC 1-2.3-76 requires submission of these isolates within (3) business days of isolation.
- Appropriate agar medium slant in tube with screw-cap tightened or other similarly approved commercial transport medium.
- Shipping boxes/containers with appropriate shipping labels, commercially available.
- Label the culture tube with a minimum of the patient's name, date of birth and date of organism isolation.
A minimum of two unique patient identifiers, (i.e. first/last name) DOB, MRN) must be indicated on the isolate tube. Unique identifiers must match what is entered on the submission form/LimsNet if available.
- Check the expiration date on the tube to ensure the product is acceptable and will continue to be acceptable once received at the IDOH Laboratory.
- Complete a Reference Bacteriology Culture Identification request form for each specimen with the following information:
- Patient name, address, age, gender, onset, physician name, and diagnosis.
- Culture isolation source, date isolated, date submitted, organism suspected.
- Submitting facility information-clinic name, address, phone number, fax number, contact name, and email address (if available).
- Relevant comments which may include patient history or travel history.
- Isolate Handling: Infectious agent, Biosafety level 2.
- Contact IDOH Epidemiology SID 317-234-6312 or 317-233-7336 if necessary.
- Package according to Category B UN3373 guidelines.
- The completed submission/request form may then be wrapped around the sealed inner container and together placed securely into the outer shipping container.
- Do not send culture isolates on petri plates if submitting by mail.
- Clearly label the outer container with the senders name/address and recipients name/address.
- Transport Temperature: Ambient conditions
Indiana Department of Health
550 West 16th Street
Indianapolis, IN. 46202
Reporting Method: Fax, phone, email, or Mail
TAT: 3-16 business days.
Test Referral. Cultures identified as Listeria monocytogenes will be sent to the CDC for confirmation and typing.